|제목||The roles of NF-κB and ROS in regulation of pro-inflammatory mediators…||작성일||18-02-20 17:10|
- 2017.07_The roles of NF-κB and ROS1세부.pdf (706.9K) 66회 다운로드 DATE : 2018-02-22 16:32:04
The roles of NF-κB and ROS in regulation of pro-inflammatory mediators of inflammation induction in LPS-stimulated zebrafish embryos.
Eun-Yi Ko, Su-Hyeon Cho, Seung-Hae Kwon, Chi-Yong Eom, Myeong Seon Jeong, WonWoo Lee, Seo-Young Kim, Soo-Jin Heo, Ginnae Ahn,
Kang Pa Lee, You-Jin Jeon, Kil-Nam Kim
Fish and Shellfish Immunology 2017, 68, 525-529
In this study, the roles of reactive oxygen species (ROS) and NF-κB on inflammation induction in lipopolysaccharide (LPS)-stimulated zebrafish embryos were evaluated using N-acetyl-l-cysteine (NAC) and pyrrolidine dithiocarbamate (PDTC), specific inhibitors of ROS and NF-κB, respectively. LPS-stimulated zebrafish embryos showed increasing production of NO and ROS and expression of iNOS and COX-2 protein, compared to a control group without LPS. However, NAC significantly inhibited production of NO and ROS and markedly suppressed expression of iNOS and COX-2 protein in LPS-stimulated zebrafish embryos. The mRNA expressions of NF-κB such as p65NF-κB and IκB-A were significantly increased after LPS stimulation, whereas PDTC attenuated mRNA expression of NF-κB. PDTC also inhibited production of NO and reduced expression of iNOS and COX-2 protein in LPS-stimulated zebrafish embryos. Taken together, these results indicated that LPS increases pro-inflammatory mediators in zebrafish embryos through ROS and NF-κB regulation.
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