제목 The roles of NF-κB and ROS in regulation of pro-inflammatory mediators… 작성일 18-02-20 17:10
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The roles of NF-κB and ROS in regulation of pro-inflammatory mediators of inflammation induction in LPS-stimulated zebrafish embryos.

Eun-Yi Ko, Su-Hyeon Cho, Seung-Hae Kwon, Chi-Yong Eom, Myeong Seon Jeong, WonWoo Lee, Seo-Young Kim, Soo-Jin Heo, Ginnae Ahn,

Kang Pa Lee, You-Jin Jeon, Kil-Nam Kim

Fish and Shellfish Immunology 2017, 68, 525-529

Abstract

In this study, the roles of reactive oxygen species (ROS) and NF-κB on inflammation induction in lipopolysaccharide (LPS)-stimulated zebrafish embryos were evaluated using N-acetyl-l-cysteine (NAC) and pyrrolidine dithiocarbamate (PDTC), specific inhibitors of ROS and NF-κB, respectively. LPS-stimulated zebrafish embryos showed increasing production of NO and ROS and expression of iNOS and COX-2 protein, compared to a control group without LPS. However, NAC significantly inhibited production of NO and ROS and markedly suppressed expression of iNOS and COX-2 protein in LPS-stimulated zebrafish embryos. The mRNA expressions of NF-κB such as p65NF-κB and IκB-A were significantly increased after LPS stimulation, whereas PDTC attenuated mRNA expression of NF-κB. PDTC also inhibited production of NO and reduced expression of iNOS and COX-2 protein in LPS-stimulated zebrafish embryos. Taken together, these results indicated that LPS increases pro-inflammatory mediators in zebrafish embryos through ROS and NF-κB regulation.

DOI: 10.1016/j.fsi.2017.07.041

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